南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测          下载免费PDF全文

王飞  丛柏林  张朝晖  杨黄浩 《极地研究》2020,32(1):68-81

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。  相似文献   

察尔汗盐湖西北部典型采区地下卤水赋存状态的地面核磁探测与数值模拟研究          下载免费PDF全文

张光武  易磊  高泽永  韩凤清  陈富洪  马喆  卢晓航  刘小宝  韩光  袁文虎  刘溪溪 《盐湖研究》2020,28(1)

察尔汗盐湖地下晶间卤水蕴藏了宝贵的盐湖矿产资源,如何确定地下卤水的赋存规律以制定科学合理的卤水开采和补给方案,正成为盐湖资源大规模可持续利用亟需解决的重要问题。本文以察尔汗盐湖西北部的典型采卤区作为研究区域,1)利用新型的地面核磁共振找水仪,通过在研究区布设两条十字交叉的测线,探测并反演解译测区地下60米深度内的卤水静态赋存形式,2)结合OpenGeoSys(OGS)多物理场耦合地下水数值模拟软件,对测区地下卤水的动态运移形式进行数值模拟计算。地面核磁探测结果表明研究区含水量整体偏低,最大含水量约0.8%,平均含水量约0.4%。测点结果给出的含水层位分界面与邻近钻孔的地层层位分界面的一致性较高,浅部的层位误差在10%以下,达到了0.5米的精度。考虑到测区南北两边均有采卤渠正在汲取地下卤水,且在测区中心存在两条废弃的采卤渠,OGS地下水数值模拟研究结果表明采卤渠造成测区浅部卤水较大空缺,与研究区浅部含水量总体偏低的探测特征一致。研究结果表明地面核磁共振技术能准确探测卤水当前的赋存状态,地下水运移模拟技术可以深入认识采补活动引起卤水赋存状态的变化规律。探测和模拟结合的联合研究是确定卤水赋存规律的重要手段,研究成果可为盐湖资源合理开采高效利用提供基础资料和相关科学支撑。  相似文献   

A modified numerical model for moisture-salt transport in unsaturated sandy soil under evaporation     

Wei Wen  YuanMing Lai  ZheMin You 《寒旱区科学》2020,12(3):125-133

Soil salinization, caused by salt migration and accumulation underneath the soil surface, will corrode structures. To analyze the moisture-salt migration and salt precipitation in soil under evaporation conditions, a mathematical model consisting of a series of theoretical equations is briefly presented. The filling effect of precipitated salts on tortuosity factor and evaporation rate are taken into account in relevant equations. Besides, a transition equation to link the solute transport equation before and after salt precipitation is proposed. Meanwhile, a new relative humidity equation deduced from Pitzer ions model is used to modify the vapor transport flux equation. The results show that the calculated values are in good agreement with the published experimental data, especially for the simulation of volume water content and evaporation rate of Toyoura sand, which confirm the reliability and applicability of the proposed model.  相似文献   

A COMPARISON OF EASTERN AND WESTERN HONG KONG PHYTOPLANKTON FROM WEEKLY SAMPLES (1997-1999)     

唐森铭 《中国海洋湖沼学报》2002,(1)

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｍａｉｎｐｕｒｐｏｓｅｏｆｔｈｉｓｓｔｕｄｙｗａｓｔｏｄｅｔｅｒｍｉｎｅｔｈｅｍａｇｎｉｔｕｄｅａｎｄｆｒｅｑｕｅｎｃｙｏｆｈａｒｍｆｕｌｄｉａｔｏｍａｎｄｄｉｎｏｆｌａｇｅｌｌａｔｅｏｕｔｂｒｅａｋｓｉｎＨｏｎｇＫｏｎｇ’ｓｗｅｓｔｅｒｎａｎｄｅａｓｔｅｒｎｗａｔｅｒｓ (ｅ .ｇ.ＬａｍｍａＳｔｒａｉｔｓａｎｄＰｏｒｔＳｈｅｌｔｅｒｒｅｓｐｅｃｔｉｖｅｌｙ)ｆｏｒｔｈｅｐｅｒｉｏｄＪａｎｕａｒｙ 1 997ｔｏＤｅｃｅｍｂｅｒ1 999.Ｔｈｉｓｗａｓｄｏｎｅｉｎｏｒｄｅｒｔｏｄ…  相似文献   

Nutrient transports in a Swedish estuary     

Wayne Wilmot  Peter Toll  Björn Kjerfve 《Estuarine, Coastal and Shelf Science》1985,21(2):161-184

Himmerfjärd is a Swedish estuary bordering on the Baltic. The estuary lacks astronomical tides and its circulation is driven by winds and freshwater runoff. Because of a tertiary sewage treatment plant located at its inner end, the estuary is becoming increasingly eutrophic. A field study was carried out for a 78-day period in late summer and early fall of 1977 to determine rates of nutrient transport and to construct nutrient budgets. Since physical parameters (current velocity, temperature, salinity, winds and water level changes) were measured more frequently than nutrients (phosphate, nitrate, nitrite and ammonium) it was necessary to develop a suitable method to calculate nutrient flux time series and net nutrient fluxes. Over the study period, Himmerfjärd imported phosphorus and exported nitrogen. Direction of nutrient fluxes and changes in flux direction were consistent with the structure of the baroclinic currents.  相似文献   

NaCl胁迫下杂交稻农艺性状配合力及遗传力分析     

方良俊 《广东海洋大学学报》2006,26(3):91-94

采用4×4不完全双列杂交设计,在NaCl胁迫下对杂交水稻主要农艺性状进行了配合力和遗传力分析.结果表明,在调查的10个性状中有4个性状受NaCl胁迫影响差异较大,母本的千粒重及父本的粒厚一般配力差异显著,杂交组合的株高、粒厚、结实率及千粒重特殊配合力差异极显著,其中杂交组合N28S×中花14具有较高的配合力,受NaCl胁迫影响较低。株高及千粒重的广义遗传力和狭义遗传力均较高,可作为杂交水稻耐盐性的选育依据。  相似文献   

STUDY ON DEVELOPMENT STRATEGY OF SHIPPING CENTERS AND TRANSPORTATION NETWORKS IN THE YELLOW SEA RIM IN THE YELLOW SEA RIM     

JINFeng-jun 《中国地理科学(英文版)》2003,13(2):97-103

It is very important to establish cooperative mechanism to guarantee all members to develop their e-conomies in the Yellow Sea Rim. In this paper, the development strategies ofshipplng centers and transportation networkare discussed based on economic giobalization tendency. The results argue that a united transportation network should bebuilt in order to promote the economic competition of Northeast Asia in the world. As a key component of the economiccooperation, a hierarchical shipping centers network should be established with Hong Kong, Shanghai, Pusan, Koho,and Tokyo as cores. The authorities of China, Japan, R. O. Korea and D. P. B. Korea should make more efforts tobuild a set of cooperation institutions based on raising the transportation efficiency.  相似文献   

^226Ra and ^228Ra in the Seawater of the Western Yellow Sea     

MEN Wu )  WEI Hao )  and LIU Guangshan )  * ) College of Oceanography  Environmental Science  Xiamen University  State Key Laboratory of Marine Environmental Science 《中国海洋大学学报(英文版)》2006,5(3):228-234

1Introduction Therearefourradiumisotopesinnature,ofwhich224Ra,226Raand228Rahavebeenwidelyinvestigated inmarineresearch,butthespecificactivityof223Rais toolowtobemeasured,therefore,itsapplicationsarelimited(Moore,1969;Broeckeretal.,1976;KuandLin,1976;Nozak…  相似文献   

Characters of the Pco2 and CO2 Flux in the East China Sea in Autumn     

纪雷  崔鹤  辛淑平  王军  张龙军  陆贤昆 《中国海洋湖沼学报》2003,21(2):180-186

Pco2 of air and seawater samples from the East China Sea(ECS) were measured in situ in autumn, 1994,Ocean currents,terrestrial fluviation,biological activities,etc.,Pco2 char-acters in air and seawater were investigated,CO2 flux and its character in the East China Sea are discussed on the basis of the Pco2 profiles of air and seawater,It was clear that the nearshore was the source of CO2;and tht the oulter sea area was the sink of CO2; and that the shelf area of the EXS is a net sink for atmospheric CO2 in autumn.  相似文献   

南海北缘北东东向构造活动性研究     

任镇寰  任奔滔  罗振暖  陈益明  钟贻军 《大地测量与地球动力学》1996,(2)

从地形地貌特征、重磁地球物理场、深部构造与断裂构造以及地震活动、震源机制等多方面论述北东东向构造是南海北缘的主要活动构造。对于长期以来认为新华夏系北东向构造是该区主要活动构造的观点来说，这是一种新的学术思想。  相似文献